| Development of a monoclonal antibody against cytoplasmic polyhedrosis virus infecting Indian saturniidae silkworms | |
| अन्वेषक | Dr. A. K. Ghosh, Ms. Uma Sinha |
| Patent | Filed |
| According to this invention is provided a process for obtaining an anti-polyhedrin monoclonal antibody comprising the steps of isolating the 10th RNA segment form polyhedra of infected larvae converting the 10th RNA segment to cDNA followed by cloning and sequencing the same, selecting the protein coding region of segment 10 and obtaining the His-tagged recombinant polyhedrin protein immunizing a subject with said protein, obtaining hybrid cells from spleen cells and myeloma cells of the immunized subject, isolating single antibody secreting cells and purifying the secreted antibody to obtain anti-polyhedrin monoclonal antibody. According to this invention is further provided an anti-polyhedrin monoclonal antibody and the use thereof for the detecting of cytoplasmic polyhedrosis virus. | |
| प्रभारी प्राध्यापक, IPR & IR प्रायोजित शोध एवं औद्योगिक सलाहकारिता भारतीय प्रौद्योगिकी संस्था खड़गपुर 721302 भारत ई-मेल: ipr @ sric.iitkgp.ernet.in |
संकायाध्यक्ष प्रायोजित शोध एवं औद्योगिक सलाहकारिता भारतीय प्रौद्योगिकी संस्था खड़गपुर 721302 भारत ई-मेल: deansr @ hijli.iitkgp.ernet.in |